Thrombin-like enzyme from the venom of Bitis gabonica. Purification, properties, and coagulant actions.
نویسندگان
چکیده
منابع مشابه
Thrombin - like Enzyme from the Venom of Bitis gubonicu
Gabonase, an enzyme which acts on fibrinogen and factor XI11 in uniquely thrombin-like ways, was purified to electrophoretic homogeneity from the venom of Bitis gabonica. On sodium dodecyl sulfate-polyacrylamide electrophoresis, the reduced protein behaved as a single chain with Mr = 30,600. The enzyme contains 20.6% carbohydrate, no free sulfhydryl groups and hence, from amino acid analysis, f...
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The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...
متن کاملBitis gabonica venom. The amino acid sequence of phospholipase A.
Bitis gabonica phospholipase A contains 118 amino acid residues in a single chain and is cross-linked by six disulfide bridges. Cyanogen bromide cleavage, and tryptic and chymotryptic digests provided the necessary overlapping peptides allowing the derivation of the complete amino acid sequence. In its primary structure the enzyme is more closely related to porcine pancreatic phospholipase A th...
متن کاملEffect of gaboon viper (Bitis gabonica) venom on blood coagulation, platelets, and the fibrinolytic enzyme system.
The action of the venom of the gaboon viper (Bitis gabonica) on blood coagulation, platelets, and the fibrinolytic enzyme system was studied. The results confirm that the venom of Bitis gabonica has a marked anticoagulant action in vitro. The venom appears to impair clot formation by a direct proteolytic action on fibrinogen, releasing soluble breakdown products.
متن کاملPurification and Functional Characterisation of Rhiminopeptidase A, a Novel Aminopeptidase from the Venom of Bitis gabonica rhinoceros
BACKGROUND Snake bite is a major neglected public health issue within poor communities living in the rural areas of several countries throughout the world. An estimated 2.5 million people are bitten by snakes each year and the cost and lack of efficacy of current anti-venom therapy, together with the lack of detailed knowledge about toxic components of venom and their modes of action, and the u...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84456-3